Summary: A new study introduces a novel bioluminescence imaging technique for observing oxygen movement in mouse brains. This method, inspired by firefly proteins, reveals real-time, widespread patterns of oxygen distribution, offering insights into conditions like hypoxia caused by strokes or heart attacks.
It further explores how sedentary lifestyles could increase Alzheimer’s risk by detecting “hypoxic pockets” or areas of temporary oxygen deprivation. This research paves the way for better understanding diseases associated with brain hypoxia and testing therapeutic interventions.
Key Facts:
- A novel bioluminescence imaging technique now allows scientists to observe oxygen movement in the brain, providing real-time, detailed views.
- The method shows that areas of the brain can experience temporary oxygen deprivation, known as “hypoxic pockets,” which are more common in sedentary states and could be linked to an increased risk of Alzheimer’s disease.
- This research, bridging work from the University of Rochester and the University of Copenhagen, can revolutionize our understanding of diseases associated with brain hypoxia and pave the way for new therapeutic interventions.
Source: University of Copenhagen
The human brain consumes vast amounts of energy, which is almost exclusively generated from a form of metabolism that requires oxygen. Consequently, the efficient and timely allocation and delivery of oxygen is critical to healthy brain function, however, the precise mechanics of this process have largely remained hidden from scientists.
A new bioluminescence imaging technique, described today in the journal Science, has created highly detailed, and visually striking, images of the movement of oxygen in the brains of mice.
The method, which can be easily replicated by other labs, will enable researchers to more precisely study forms of hypoxia, such as the denial of oxygen to parts of the brain that occurs during a stroke or heart attack. It is already providing insight into why a sedentary lifestyle increases risk for diseases like Alzheimer’s.
“This research demonstrates that we can monitor changes in oxygen concentration continuously and in a wide area of the brain,” says Maiken Nedergaard, co-director of the Center for Translational Neuromedicine, which is based at both the University of Rochester and the University of Copenhagen.
“This provides us a with a more detailed picture of what is occurring in the brain in real time, allowing us to identify previously undetected areas of temporary hypoxia, which reflect changes in blood flow that can trigger neurological deficits” says Maiken Nedergaard.
Fireflies and serendipitous science
The new method employs luminescent proteins, chemical cousins of the bioluminescent proteins found in fireflies. These proteins, which have been used in cancer research, employ a virus that delivers instructions to cells to produce a luminescent protein in the form of an enzyme. When the enzyme encounters its substrate called furimazine, the chemical reaction generates light.
Like many important scientific discoveries, employing this process to image oxygen in the brain was stumbled upon by accident. Felix Beinlich, Assistant Professor at the Center for Translational Neuroscience at the University of Copenhagen, had originally intended to use luminescent proteins to measure calcium activity in the brain. It became clear there was an error in the protein production, causing a months-long research delay.
While Felix Beinlich waited for a new batch from the manufacturer, he decided to move forward with the experiments to test and optimize the monitoring systems. The virus was used to deliver enzyme-producing instructions to astrocytes, ubiquitous support cells in the brain that maintain the health and signaling functions of neurons, and the substrate was injected directly into the brain.
The recordings revealed activity, identified by a fluctuating intensity of bioluminescence, something that the researchers suspected, and would later confirm, reflected the presence and concentration of oxygen. “The chemical reaction in this instance was oxygen dependent, so when there is the enzyme, the substrate, and oxygen, the system starts to glow,” says Felix Beinlich.
While existing oxygen monitoring techniques provide information about a small area of the brain, the researchers observed, in real time, the entire cortex of the mice. The intensity of the bioluminescence corresponded with the concentration of oxygen, which the researchers demonstrated by changing the amount of oxygen in the air the animals were breathing.
Changes in light intensity also corresponded with sensory processing. For example, when the mice’s whiskers were stimulated with a puff of air, the researchers could see the corresponding sensory region of the brain light up.
“Hypoxic pockets” could point to Alzheimer’s risk
The brain cannot survive long without oxygen, a concept demonstrated by the neurological damage that quickly follows a stroke or heart attack. But what happens when small parts of the brain are denied oxygen for brief periods?
This question was not even being asked by researchers until the team in the Nedergaard lab began to look closely at the new recordings. While monitoring the mice, the researchers observed that specific tiny areas of the brain would intermittently go dark, sometimes for several seconds, meaning that the oxygen supply was cut off.
Oxygen is circulated throughout the brain via a vast network of arteries and smaller capillaries–or microvessels–which permeate brain tissue.
Through a series of experiments, the researchers were able to determine that oxygen was being denied due to capillary stalling, which occurs when white blood cells temporarily block microvessels and prevent the passage of oxygen carrying red blood cells.
These areas, which the researchers named “hypoxic pockets,” were more prevalent in the brains of mice during a resting state, compared to when the animals were active. Capillary stalling is believed to increase with age and has been observed in models of Alzheimer’s disease.
“The door is open to study a range of diseases associated with hypoxia in the brain, including Alzheimer’s, vascular dementia, and long COVID, and how a sedentary lifestyle, aging, hypertension, and other factors contribute to these diseases,” says Maiken Nedergaard and adds:
“It also provides a tool to test different drugs and types of exercise that improve vascular health and slow down the road to dementia.”
Additional authors include Antonios Asiminas with the University of Copenhagen, Hajime Hirase with the University of Rochester, Verena Untiet, Zuzanna Bojarowska, Virginia Plá, and Björn Sigurdsson with the University of Copenhagen, and Vincenzo Timmel, Lukas Gehrig, and Michael H. Graber with the University of Applied Sciences and Arts Northwestern Switzerland.
Funding: The study was supported with funding from the National Institute of Neurological Disorders and Stroke, the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation, the Novo Nordisk Foundation, the Lundbeck Foundation, Independent Research Fund Denmark, and the US Army Research Office.
About this neuroscience research news
Author: Liva Polack
Source: University of Copenhagen
Contact: Liva Polack – University of Copenhagen
Image: The image is credited to Neuroscience News
Original Research: Closed access.
“Oxygen imaging of hypoxic pockets in the mouse cerebral cortex” by Maiken Nedergaard et al. Science
Abstract
Oxygen imaging of hypoxic pockets in the mouse cerebral cortex
Consciousness is lost within seconds upon cessation of cerebral blood flow. The brain cannot store oxygen, and interruption of oxidative phosphorylation is fatal within minutes. Yet only rudimentary knowledge exists regarding cortical partial oxygen tension (Po2) dynamics under physiological conditions.
Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for Po2 imaging.
In awake behaving mice, we uncover the existence of spontaneous, spatially defined “hypoxic pockets” and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest.
The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.
